Characterisation of a Putative Agent of Horizontal Gene Transfer Encoded by a Bacterial Prophage Region
Y3877831
1 Introduction
MuGam is a orthologue of eukaryotic proteins Ku70/Ku80, which are involved in recognizing double-strand DNA prior to repair, as well as DNA transposition. In Haemophilus influenzae, Gam (HiGam) inhibits digestion of Mu phage DNA by nucleases (d’Adda di Fagagna, Weller, Doherty, et al., 2003).
The aim of this report is to characterise HiGam by processing the data obtained from the following experiments:
- Polymerase chain reaction (PCR)
- Electrophoretic mobility shift assay (EMSA)
- Size-exclusion chromatography (SEC)
- Analytical ultracentrifugation (AUC) ???
2 Requirements
Required software and packages are outlined in the README.
3 Preparing Data
4 Analysis
4.1 PCR
Diagnostic PCR was used to amplify the H. influenzae homologues, and to test for their transcription relative to the Genomic DNA (gDNA) control. Amplicon sizes were established using DNA ladder molecular weight (MW) standards. Results identified the transcription of HiA, HiB, HiGam, HiA-HiB and HiB-HiGam in H. influenzae. Figure @(fig:pcr-fig) shows
\[\Large \color{#8D9FCA} y \color{black} = \color{#E789C2} m \color{#66c2a4} x \color{black} + \color{#FFD82F} c\]
4.2 Electrophoretic Mobility Shift Assay (EMSA) & Binding Affinity
Electrophoretic mobility shift assay (EMSA) was used to examine whether Im9-HiGam–DNA had an affinity for closed circular DNA or linear DNA. Im9-HiGam was observed to bind preferentially to linear DNA with a KD of approximately 0.55 (4.1). Results estimate Im9-HiGam has a binding dimeric footprint of 22 base pairs.
Figure 4.1: (fig:emsa-fig)
4.3 Size Exclusion Chromatography (SEC)
Size exclusion chromatography (SEC) was used to explore the molecular weight and oligomeric state of Im9-HiGam. 7 chromatograms of known MW were plotted and a linear regression was performed to determine the relationship between elution volume and molecular weight. The SEC data showed a peak for both wavelengths, with a MW of approximately 58853.86 Daltons for A254 and 77703.42 Daltons for A280. This gives Im9-HiGam has an estimated dimeric footprint of approximately 28.56 base pairs.
4.3.1
SEC analysis
SEC Standards
SEC dataset
| Volume | a254 | a280 |
|---|---|---|
| 0.00 | 0.10 | 0.18 |
| 0.02 | 0.20 | 0.18 |
| 0.05 | 0.07 | -0.01 |
| 0.07 | -0.04 | -0.04 |
| 0.10 | -0.02 | -0.20 |
| 0.12 | -0.11 | 0.10 |
| 0.14 | 0.07 | -0.09 |
| 0.17 | -0.03 | 0.06 |
| 0.19 | -0.06 | -0.36 |
| 0.22 | 0.07 | -0.15 |
| 0.24 | 0.14 | -0.33 |
| 0.26 | -0.12 | -0.10 |
| 0.29 | 0.19 | 0.14 |
| 0.31 | 0.02 | 0.04 |
| 0.34 | 0.05 | 0.12 |
| 0.36 | 0.03 | 0.19 |
| 0.38 | -0.02 | -0.32 |
| 0.41 | 0.12 | 0.21 |
| 0.43 | -0.13 | 0.01 |
| 0.46 | 0.08 | -0.07 |
| 0.48 | -0.11 | 0.15 |
| 0.50 | -0.06 | -0.03 |
| 0.53 | -0.11 | -0.02 |
| 0.55 | 0.01 | -0.02 |
| 0.58 | -0.15 | -0.04 |
| 0.60 | 0.10 | -0.03 |
| 0.62 | 0.13 | -0.12 |
| 0.65 | -0.17 | 0.32 |
| 0.67 | 0.05 | 0.04 |
| 0.70 | 0.19 | 0.27 |
| 0.72 | 0.12 | -0.21 |
| 0.74 | 0.20 | -0.25 |
| 0.77 | 0.08 | 0.06 |
| 0.79 | -0.14 | 0.18 |
| 0.82 | -0.03 | 0.04 |
| 0.84 | -0.17 | -0.13 |
| 0.86 | 0.10 | 0.08 |
| 0.89 | 0.07 | -0.04 |
| 0.91 | 0.02 | 0.35 |
| 0.94 | 0.16 | -0.02 |
| 0.96 | 0.00 | 0.09 |
| 0.98 | 0.03 | 0.01 |
| 1.01 | -0.10 | 0.12 |
| 1.03 | -0.16 | -0.23 |
| 1.06 | 0.13 | -0.09 |
| 1.08 | -0.19 | -0.04 |
| 1.11 | 0.11 | -0.14 |
| 1.13 | 0.03 | 0.00 |
| 1.15 | 0.04 | -0.05 |
| 1.18 | -0.06 | 0.01 |
| 1.20 | -0.18 | 0.02 |
| 1.23 | 0.05 | 0.15 |
| 1.25 | 0.01 | 0.02 |
| 1.27 | -0.14 | -0.27 |
| 1.30 | 0.15 | -0.19 |
| 1.32 | -0.17 | -0.29 |
| 1.35 | -0.02 | -0.18 |
| 1.37 | 0.14 | -0.15 |
| 1.39 | 0.02 | 0.10 |
| 1.42 | -0.06 | -0.27 |
| 1.44 | 0.01 | 0.03 |
| 1.47 | -0.13 | 0.05 |
| 1.49 | 0.07 | -0.32 |
| 1.51 | -0.07 | -0.21 |
| 1.54 | -0.15 | -0.04 |
| 1.56 | 0.01 | -0.02 |
| 1.59 | -0.01 | 0.11 |
| 1.61 | -0.13 | -0.16 |
| 1.63 | 0.03 | 0.19 |
| 1.66 | 0.13 | 0.19 |
| 1.68 | -0.18 | -0.09 |
| 1.71 | 0.03 | 0.07 |
| 1.73 | 0.15 | -0.02 |
| 1.75 | 0.03 | 0.14 |
| 1.78 | -0.01 | 0.18 |
| 1.80 | 0.14 | -0.19 |
| 1.83 | 0.11 | 0.26 |
| 1.85 | -0.15 | -0.14 |
| 1.87 | -0.17 | -0.24 |
| 1.90 | 0.11 | -0.03 |
| 1.92 | -0.21 | 0.17 |
| 1.95 | 0.12 | 0.29 |
| 1.97 | 0.06 | -0.28 |
| 1.99 | 0.19 | -0.03 |
| 2.02 | 0.15 | -0.06 |
| 2.04 | -0.10 | 0.21 |
| 2.07 | -0.21 | -0.19 |
| 2.09 | 0.06 | 0.08 |
| 2.11 | 0.05 | -0.02 |
| 2.14 | 0.17 | -0.34 |
| 2.16 | -0.13 | -0.24 |
| 2.19 | 0.17 | 0.10 |
| 2.21 | -0.10 | 0.08 |
| 2.23 | 0.06 | -0.04 |
| 2.26 | -0.14 | 0.00 |
| 2.28 | 0.09 | 0.10 |
| 2.31 | -0.04 | 0.18 |
| 2.33 | 0.03 | 0.12 |
| 2.35 | -0.03 | 0.21 |
| 2.38 | -0.19 | 0.06 |
| 2.40 | 0.19 | 0.33 |
| 2.43 | -0.06 | 0.24 |
| 2.45 | 0.12 | 0.28 |
| 2.47 | -0.12 | 0.07 |
| 2.50 | 0.16 | 0.19 |
| 2.52 | -0.14 | 0.21 |
| 2.55 | 0.17 | 0.02 |
| 2.57 | -0.07 | 0.12 |
| 2.59 | 0.17 | 0.01 |
| 2.62 | 0.14 | -0.06 |
| 2.64 | 0.10 | 0.18 |
| 2.67 | -0.05 | 0.17 |
| 2.69 | 0.10 | 0.03 |
| 2.71 | 0.11 | 0.24 |
| 2.74 | -0.10 | -0.08 |
| 2.76 | 0.03 | -0.03 |
| 2.79 | 0.05 | -0.01 |
| 2.81 | -0.13 | 0.33 |
| 2.83 | 0.08 | 0.03 |
| 2.86 | 0.15 | -0.02 |
| 2.88 | -0.02 | 0.32 |
| 2.91 | -0.20 | 0.35 |
| 2.93 | 0.00 | -0.07 |
| 2.95 | 0.07 | 0.18 |
| 2.98 | -0.12 | 0.06 |
| 3.00 | -0.01 | 0.10 |
| 3.03 | -0.03 | 0.06 |
| 3.05 | -0.14 | -0.16 |
| 3.08 | -0.02 | 0.13 |
| 3.10 | 0.00 | -0.11 |
| 3.12 | -0.10 | -0.20 |
| 3.15 | -0.13 | -0.07 |
| 3.17 | 0.02 | -0.13 |
| 3.20 | 0.13 | 0.21 |
| 3.22 | 0.19 | -0.18 |
| 3.24 | 0.14 | -0.08 |
| 3.27 | 0.18 | 0.24 |
| 3.29 | -0.16 | -0.31 |
| 3.32 | 0.05 | -0.23 |
| 3.34 | -0.02 | 0.05 |
| 3.36 | -0.02 | -0.11 |
| 3.39 | -0.02 | 0.12 |
| 3.41 | 0.02 | -0.21 |
| 3.44 | 0.04 | 0.12 |
| 3.46 | -0.10 | 0.01 |
| 3.48 | 0.01 | 0.03 |
| 3.51 | 0.09 | 0.11 |
| 3.53 | 0.09 | -0.03 |
| 3.56 | 0.12 | -0.08 |
| 3.58 | 0.19 | -0.06 |
| 3.60 | 0.10 | 0.06 |
| 3.63 | -0.01 | -0.12 |
| 3.65 | 0.19 | -0.02 |
| 3.68 | -0.11 | -0.16 |
| 3.70 | -0.06 | -0.08 |
| 3.72 | -0.03 | -0.39 |
| 3.75 | 0.00 | -0.11 |
| 3.77 | -0.07 | 0.00 |
| 3.80 | 0.11 | -0.16 |
| 3.82 | 0.13 | -0.07 |
| 3.84 | 0.10 | -0.22 |
| 3.87 | -0.16 | -0.20 |
| 3.89 | 0.16 | -0.08 |
| 3.92 | 0.06 | 0.32 |
| 3.94 | 0.11 | 0.13 |
| 3.96 | -0.05 | -0.12 |
| 3.99 | -0.17 | -0.18 |
| 4.01 | 0.11 | -0.04 |
| 4.04 | -0.02 | -0.31 |
| 4.06 | -0.05 | -0.02 |
| 4.08 | 0.11 | 0.02 |
| 4.11 | 0.13 | -0.32 |
| 4.13 | 0.15 | -0.16 |
| 4.16 | 0.11 | -0.28 |
| 4.18 | 0.03 | -0.03 |
| 4.20 | 0.06 | 0.06 |
| 4.23 | -0.01 | 0.15 |
| 4.25 | -0.03 | 0.16 |
| 4.28 | -0.01 | 0.15 |
| 4.30 | -0.15 | 0.07 |
| 4.32 | 0.20 | -0.01 |
| 4.35 | -0.15 | -0.15 |
| 4.37 | -0.02 | 0.12 |
| 4.40 | 0.16 | 0.10 |
| 4.42 | -0.13 | -0.23 |
| 4.44 | -0.05 | -0.13 |
| 4.47 | -0.15 | -0.29 |
| 4.49 | -0.03 | 0.03 |
| 4.52 | -0.01 | 0.07 |
| 4.54 | -0.03 | -0.19 |
| 4.56 | 0.12 | -0.04 |
| 4.59 | -0.20 | -0.01 |
| 4.61 | 0.12 | 0.27 |
| 4.64 | 0.04 | -0.21 |
| 4.66 | -0.12 | -0.18 |
| 4.68 | 0.03 | -0.15 |
| 4.71 | -0.08 | -0.05 |
| 4.73 | -0.06 | 0.09 |
| 4.76 | -0.06 | 0.12 |
| 4.78 | -0.19 | -0.01 |
| 4.80 | 0.02 | -0.35 |
| 4.83 | 0.02 | 0.33 |
| 4.85 | 0.09 | 0.25 |
| 4.88 | -0.14 | -0.10 |
| 4.90 | 0.15 | 0.18 |
| 4.92 | 0.15 | 0.12 |
| 4.95 | -0.10 | 0.02 |
| 4.97 | -0.03 | -0.07 |
| 5.00 | -0.05 | -0.01 |
| 5.02 | -0.13 | -0.03 |
| 5.05 | 0.04 | 0.23 |
| 5.07 | 0.09 | -0.07 |
| 5.09 | 0.09 | -0.06 |
| 5.12 | 0.02 | 0.04 |
| 5.14 | -0.15 | -0.15 |
| 5.17 | -0.11 | 0.02 |
| 5.19 | -0.03 | 0.31 |
| 5.21 | -0.20 | 0.01 |
| 5.24 | 0.06 | -0.15 |
| 5.26 | 0.00 | -0.24 |
| 5.29 | 0.09 | 0.21 |
| 5.31 | -0.08 | 0.05 |
| 5.33 | 0.07 | -0.12 |
| 5.36 | 0.11 | 0.09 |
| 5.38 | -0.12 | -0.06 |
| 5.41 | -0.03 | -0.14 |
| 5.43 | -0.05 | -0.03 |
| 5.45 | 0.13 | -0.18 |
| 5.48 | -0.06 | -0.03 |
| 5.50 | 0.20 | 0.15 |
| 5.53 | -0.05 | 0.20 |
| 5.55 | 0.05 | -0.08 |
| 5.57 | 0.14 | 0.05 |
| 5.60 | 0.06 | -0.12 |
| 5.62 | -0.12 | 0.18 |
| 5.65 | -0.16 | 0.01 |
| 5.67 | -0.11 | -0.20 |
| 5.69 | -0.16 | -0.28 |
| 5.72 | -0.11 | 0.06 |
| 5.74 | -0.16 | -0.20 |
| 5.77 | -0.07 | -0.07 |
| 5.79 | 0.01 | -0.01 |
| 5.81 | 0.16 | -0.07 |
| 5.84 | -0.12 | 0.06 |
| 5.86 | 0.12 | 0.12 |
| 5.89 | -0.07 | 0.19 |
| 5.91 | 0.02 | 0.27 |
| 5.93 | -0.10 | -0.02 |
| 5.96 | -0.12 | -0.05 |
| 5.98 | 0.18 | 0.28 |
| 6.01 | -0.15 | 0.01 |
| 6.03 | 0.12 | -0.07 |
| 6.05 | -0.16 | 0.24 |
| 6.08 | 0.02 | 0.06 |
| 6.10 | -0.01 | 0.05 |
| 6.13 | -0.08 | 0.25 |
| 6.15 | 0.09 | 0.24 |
| 6.17 | -0.01 | 0.27 |
| 6.20 | -0.03 | -0.16 |
| 6.22 | 0.07 | -0.32 |
| 6.25 | -0.19 | -0.04 |
| 6.27 | -0.19 | -0.22 |
| 6.29 | -0.08 | 0.22 |
| 6.32 | 0.17 | -0.16 |
| 6.34 | -0.08 | 0.01 |
| 6.37 | -0.11 | -0.30 |
| 6.39 | -0.12 | 0.03 |
| 6.41 | 0.03 | -0.29 |
| 6.44 | -0.12 | 0.31 |
| 6.46 | -0.08 | 0.01 |
| 6.49 | 0.13 | 0.21 |
| 6.51 | 0.05 | -0.06 |
| 6.53 | 0.16 | -0.06 |
| 6.56 | -0.13 | -0.12 |
| 6.58 | 0.17 | 0.33 |
| 6.61 | 0.13 | 0.00 |
| 6.63 | 0.12 | -0.09 |
| 6.65 | -0.18 | -0.06 |
| 6.68 | 0.21 | 0.33 |
| 6.70 | 0.12 | 0.31 |
| 6.73 | -0.11 | 0.00 |
| 6.75 | -0.17 | 0.00 |
| 6.77 | 0.16 | 0.23 |
| 6.80 | -0.03 | -0.05 |
| 6.82 | -0.09 | 0.27 |
| 6.85 | 0.18 | 0.01 |
| 6.87 | 0.17 | -0.27 |
| 6.89 | -0.09 | 0.11 |
| 6.92 | 0.01 | -0.17 |
| 6.94 | -0.17 | -0.02 |
| 6.97 | 0.19 | 0.01 |
| 6.99 | -0.06 | 0.29 |
| 7.02 | 0.01 | 0.04 |
| 7.04 | 0.11 | -0.17 |
| 7.06 | 0.02 | -0.33 |
| 7.09 | -0.01 | -0.15 |
| 7.11 | -0.07 | -0.30 |
| 7.14 | -0.05 | -0.26 |
| 7.16 | -0.05 | -0.04 |
| 7.18 | -0.12 | -0.17 |
| 7.21 | 0.14 | -0.10 |
| 7.23 | -0.08 | -0.08 |
| 7.26 | -0.16 | 0.21 |
| 7.28 | -0.03 | -0.12 |
| 7.30 | -0.16 | 0.13 |
| 7.33 | 0.18 | 0.12 |
| 7.35 | -0.19 | 0.14 |
| 7.38 | 0.00 | 0.17 |
| 7.40 | 0.08 | 0.23 |
| 7.42 | -0.18 | 0.14 |
| 7.45 | -0.03 | -0.22 |
| 7.47 | 0.04 | 0.21 |
| 7.50 | -0.04 | 0.07 |
| 7.52 | -0.14 | -0.08 |
| 7.54 | -0.08 | 0.05 |
| 7.57 | 0.16 | 0.02 |
| 7.59 | 0.04 | -0.08 |
| 7.62 | 0.01 | 0.27 |
| 7.64 | -0.17 | -0.05 |
| 7.66 | 0.06 | 0.24 |
| 7.69 | 0.05 | -0.05 |
| 7.71 | 0.15 | -0.05 |
| 7.74 | -0.11 | 0.03 |
| 7.76 | 0.17 | 0.22 |
| 7.78 | -0.18 | 0.31 |
| 7.81 | -0.04 | 0.25 |
| 7.83 | -0.08 | -0.34 |
| 7.86 | -0.19 | -0.37 |
| 7.88 | -0.08 | -0.05 |
| 7.90 | 0.17 | 0.11 |
| 7.93 | -0.20 | 0.03 |
| 7.95 | -0.01 | 0.22 |
| 7.98 | 0.18 | -0.14 |
| 8.00 | -0.12 | -0.06 |
| 8.02 | 0.08 | -0.31 |
| 8.05 | -0.04 | -0.23 |
| 8.07 | 0.16 | 0.01 |
| 8.10 | -0.18 | 0.17 |
| 8.12 | -0.16 | -0.13 |
| 8.14 | 0.06 | 0.21 |
| 8.17 | 0.08 | -0.13 |
| 8.19 | -0.04 | 0.01 |
| 8.22 | -0.04 | -0.16 |
| 8.24 | 0.15 | -0.15 |
| 8.26 | -0.18 | -0.15 |
| 8.29 | 0.06 | -0.05 |
| 8.31 | -0.21 | 0.24 |
| 8.34 | 0.12 | 0.03 |
| 8.36 | 0.02 | -0.22 |
| 8.38 | 0.10 | 0.06 |
| 8.41 | -0.09 | 0.28 |
| 8.43 | -0.18 | -0.04 |
| 8.46 | 0.03 | -0.05 |
| 8.48 | 0.06 | -0.18 |
| 8.50 | 0.10 | 0.07 |
| 8.53 | 0.08 | 0.31 |
| 8.55 | -0.03 | 0.06 |
| 8.58 | 0.19 | 0.08 |
| 8.60 | -0.08 | 0.01 |
| 8.62 | -0.03 | -0.17 |
| 8.65 | -0.10 | -0.12 |
| 8.67 | -0.17 | 0.31 |
| 8.70 | -0.13 | -0.17 |
| 8.72 | 0.07 | 0.22 |
| 8.74 | -0.08 | 0.20 |
| 8.77 | 0.22 | 0.10 |
| 8.79 | 0.12 | -0.21 |
| 8.82 | -0.11 | 0.07 |
| 8.84 | -0.02 | 0.24 |
| 8.86 | -0.19 | 0.18 |
| 8.89 | 0.16 | -0.08 |
| 8.91 | 0.02 | -0.13 |
| 8.94 | 0.17 | 0.24 |
| 8.96 | -0.12 | 0.16 |
| 8.98 | -0.10 | 0.13 |
| 9.01 | -0.12 | 0.13 |
| 9.03 | 0.01 | -0.09 |
| 9.06 | -0.02 | 0.15 |
| 9.08 | -0.10 | 0.25 |
| 9.11 | -0.06 | 0.08 |
| 9.13 | -0.01 | -0.08 |
| 9.15 | 0.05 | -0.07 |
| 9.18 | 0.16 | 0.04 |
| 9.20 | 0.11 | -0.36 |
| 9.23 | 0.05 | 0.01 |
| 9.25 | -0.10 | -0.09 |
| 9.27 | 0.15 | -0.06 |
| 9.30 | -0.19 | -0.15 |
| 9.32 | -0.10 | 0.21 |
| 9.35 | -0.13 | -0.07 |
| 9.37 | 0.06 | -0.21 |
| 9.39 | 0.09 | -0.03 |
| 9.42 | 0.19 | 0.04 |
| 9.44 | 0.15 | -0.18 |
| 9.47 | -0.19 | 0.03 |
| 9.49 | 0.13 | -0.01 |
| 9.51 | 0.09 | -0.33 |
| 9.54 | 0.14 | 0.02 |
| 9.56 | 0.17 | 0.05 |
| 9.59 | 0.01 | -0.17 |
| 9.61 | 0.01 | -0.26 |
| 9.63 | -0.09 | 0.08 |
| 9.66 | -0.03 | 0.16 |
| 9.68 | -0.06 | -0.02 |
| 9.71 | 0.07 | -0.01 |
| 9.73 | -0.04 | -0.15 |
| 9.75 | 0.05 | 0.01 |
| 9.78 | -0.10 | -0.14 |
| 9.80 | 0.00 | -0.15 |
| 9.83 | 0.02 | -0.05 |
| 9.85 | 0.10 | 0.06 |
| 9.87 | -0.07 | 0.25 |
| 9.90 | -0.01 | 0.02 |
| 9.92 | 0.11 | -0.01 |
| 9.95 | -0.16 | 0.01 |
| 9.97 | -0.09 | -0.03 |
| 9.99 | 0.18 | -0.21 |
| 10.02 | -0.01 | -0.05 |
| 10.04 | 0.18 | 0.08 |
| 10.07 | 0.04 | 0.11 |
| 10.09 | -0.12 | 0.05 |
| 10.11 | -0.05 | 0.04 |
| 10.14 | -0.13 | -0.30 |
| 10.16 | 0.08 | -0.30 |
| 10.19 | 0.06 | -0.33 |
| 10.21 | 0.00 | -0.12 |
| 10.23 | 0.16 | -0.02 |
| 10.26 | 0.04 | 0.22 |
| 10.28 | -0.18 | -0.24 |
| 10.31 | 0.00 | -0.06 |
| 10.33 | 0.03 | -0.16 |
| 10.35 | -0.13 | 0.18 |
| 10.38 | 0.14 | 0.28 |
| 10.40 | -0.10 | 0.04 |
| 10.43 | -0.08 | 0.37 |
| 10.45 | 0.15 | 0.20 |
| 10.47 | 0.00 | 0.00 |
| 10.50 | 0.01 | -0.37 |
| 10.52 | -0.17 | -0.14 |
| 10.55 | 0.10 | 0.13 |
| 10.57 | 0.14 | -0.01 |
| 10.59 | 0.06 | -0.30 |
| 10.62 | 0.14 | -0.20 |
| 10.64 | 0.09 | -0.26 |
| 10.67 | -0.02 | 0.31 |
| 10.69 | 0.06 | -0.29 |
| 10.71 | -0.04 | 0.06 |
| 10.74 | 0.17 | 0.05 |
| 10.76 | -0.10 | 0.22 |
| 10.79 | 0.15 | 0.03 |
| 10.81 | 0.08 | 0.18 |
| 10.83 | 0.20 | -0.02 |
| 10.86 | 0.05 | -0.23 |
| 10.88 | -0.11 | -0.01 |
| 10.91 | -0.10 | -0.27 |
| 10.93 | 0.06 | -0.22 |
| 10.95 | -0.07 | -0.34 |
| 10.98 | 0.07 | -0.30 |
| 11.00 | -0.16 | 0.17 |
| 11.03 | 0.15 | 0.03 |
| 11.05 | -0.19 | -0.06 |
| 11.08 | -0.09 | 0.00 |
| 11.10 | 0.05 | -0.13 |
| 11.12 | -0.04 | 0.10 |
| 11.15 | 0.12 | 0.07 |
| 11.17 | -0.15 | 0.08 |
| 11.20 | 0.08 | 0.16 |
| 11.22 | 0.18 | -0.29 |
| 11.24 | -0.13 | 0.29 |
| 11.27 | 0.00 | 0.35 |
| 11.29 | 0.14 | 0.15 |
| 11.32 | 0.17 | 0.11 |
| 11.34 | 0.15 | -0.15 |
| 11.36 | -0.04 | 0.12 |
| 11.39 | 0.04 | -0.15 |
| 11.41 | 0.02 | 0.03 |
| 11.44 | -0.09 | 0.09 |
| 11.46 | -0.19 | 0.25 |
| 11.48 | 0.02 | -0.25 |
| 11.51 | 0.08 | 0.15 |
| 11.53 | 0.14 | -0.04 |
| 11.56 | -0.04 | -0.19 |
| 11.58 | 0.04 | -0.17 |
| 11.60 | -0.08 | -0.08 |
| 11.63 | 0.03 | -0.09 |
| 11.65 | -0.14 | 0.02 |
| 11.68 | 0.01 | 0.02 |
| 11.70 | 0.09 | 0.17 |
| 11.72 | 0.04 | 0.01 |
| 11.75 | 0.18 | 0.16 |
| 11.77 | -0.07 | 0.12 |
| 11.80 | -0.14 | 0.03 |
| 11.82 | -0.09 | -0.12 |
| 11.84 | 0.05 | -0.20 |
| 11.87 | -0.02 | -0.04 |
| 11.89 | 0.12 | -0.06 |
| 11.92 | -0.18 | 0.11 |
| 11.94 | -0.07 | -0.04 |
| 11.96 | -0.05 | -0.04 |
| 11.99 | 0.06 | -0.16 |
| 12.01 | 0.05 | 0.28 |
| 12.04 | 0.21 | -0.04 |
| 12.06 | -0.03 | 0.23 |
| 12.08 | 0.19 | 0.03 |
| 12.11 | 0.14 | 0.20 |
| 12.13 | -0.01 | 0.11 |
| 12.16 | -0.05 | 0.07 |
| 12.18 | 0.13 | -0.08 |
| 12.20 | 0.10 | -0.01 |
| 12.23 | 0.10 | -0.03 |
| 12.25 | -0.17 | -0.21 |
| 12.28 | -0.19 | -0.25 |
| 12.30 | -0.05 | -0.33 |
| 12.32 | 0.03 | -0.24 |
| 12.35 | 0.04 | -0.08 |
| 12.37 | -0.01 | 0.01 |
| 12.40 | -0.02 | 0.06 |
| 12.42 | -0.01 | 0.01 |
| 12.44 | -0.03 | -0.07 |
| 12.47 | -0.12 | -0.02 |
| 12.49 | 0.16 | 0.18 |
| 12.52 | 0.08 | 0.05 |
| 12.54 | -0.01 | -0.08 |
| 12.56 | 0.21 | -0.15 |
| 12.59 | -0.01 | 0.01 |
| 12.61 | -0.12 | -0.11 |
| 12.64 | -0.09 | 0.09 |
| 12.66 | -0.19 | 0.21 |
| 12.68 | 0.11 | 0.04 |
| 12.71 | -0.09 | -0.01 |
| 12.73 | 0.11 | -0.29 |
| 12.76 | 0.19 | 0.03 |
| 12.78 | 0.04 | 0.09 |
| 12.80 | 0.19 | -0.02 |
| 12.83 | 0.13 | 0.02 |
| 12.85 | 0.08 | 0.09 |
| 12.88 | -0.01 | 0.12 |
| 12.90 | -0.07 | 0.05 |
| 12.92 | 0.13 | -0.12 |
| 12.95 | -0.12 | -0.18 |
| 12.97 | 0.08 | 0.25 |
| 13.00 | 0.00 | -0.16 |
| 13.02 | -0.01 | -0.13 |
| 13.05 | -0.12 | 0.27 |
| 13.07 | -0.16 | 0.07 |
| 13.09 | -0.03 | 0.13 |
| 13.12 | -0.14 | 0.21 |
| 13.14 | -0.07 | 0.09 |
| 13.17 | 0.07 | 0.33 |
| 13.19 | 0.04 | 0.12 |
| 13.21 | -0.10 | -0.23 |
| 13.24 | -0.14 | 0.29 |
| 13.26 | 0.04 | 0.12 |
| 13.29 | 0.07 | 0.12 |
| 13.31 | 0.10 | 0.29 |
| 13.33 | -0.09 | 0.11 |
| 13.36 | -0.17 | 0.13 |
| 13.38 | 0.11 | -0.26 |
| 13.41 | -0.12 | -0.03 |
| 13.43 | -0.03 | 0.00 |
| 13.45 | 0.14 | 0.04 |
| 13.48 | 0.11 | -0.22 |
| 13.50 | 0.13 | 0.27 |
| 13.53 | -0.04 | 0.10 |
| 13.55 | -0.13 | -0.22 |
| 13.57 | 0.19 | -0.10 |
| 13.60 | -0.16 | 0.05 |
| 13.62 | 0.10 | 0.17 |
| 13.65 | 0.10 | -0.16 |
| 13.67 | 0.11 | 0.21 |
| 13.69 | -0.18 | 0.11 |
| 13.72 | 0.17 | 0.05 |
| 13.74 | 0.17 | 0.38 |
| 13.77 | 0.03 | -0.17 |
| 13.79 | 0.20 | 0.08 |
| 13.81 | -0.17 | 0.07 |
| 13.84 | 0.15 | -0.20 |
| 13.86 | -0.12 | 0.16 |
| 13.89 | 0.00 | -0.22 |
| 13.91 | 0.19 | -0.14 |
| 13.93 | -0.12 | 0.15 |
| 13.96 | 0.07 | -0.31 |
| 13.98 | 0.02 | 0.07 |
| 14.01 | 0.10 | 0.10 |
| 14.03 | 0.00 | 0.24 |
| 14.05 | 0.18 | -0.39 |
| 14.08 | -0.08 | 0.32 |
| 14.10 | -0.04 | -0.11 |
| 14.13 | -0.15 | -0.17 |
| 14.15 | -0.18 | -0.26 |
| 14.17 | 0.15 | -0.11 |
| 14.20 | 0.00 | 0.23 |
| 14.22 | -0.19 | 0.39 |
| 14.25 | -0.14 | 0.12 |
| 14.27 | 0.13 | 0.37 |
| 14.29 | 0.01 | 0.47 |
| 14.32 | -0.09 | 0.85 |
| 14.34 | 0.33 | 0.97 |
| 14.37 | 0.18 | 1.73 |
| 14.39 | 0.65 | 2.07 |
| 14.41 | 0.92 | 2.81 |
| 14.44 | 1.61 | 3.61 |
| 14.46 | 2.56 | 4.83 |
| 14.49 | 3.95 | 5.86 |
| 14.51 | 5.78 | 7.80 |
| 14.53 | 8.70 | 9.62 |
| 14.56 | 12.19 | 11.65 |
| 14.58 | 17.27 | 13.71 |
| 14.61 | 23.22 | 16.31 |
| 14.63 | 30.75 | 19.22 |
| 14.65 | 39.16 | 21.21 |
| 14.68 | 48.40 | 24.35 |
| 14.70 | 58.13 | 26.80 |
| 14.73 | 67.83 | 28.88 |
| 14.75 | 76.94 | 31.51 |
| 14.77 | 84.94 | 32.86 |
| 14.80 | 90.78 | 33.98 |
| 14.82 | 94.07 | 34.83 |
| 14.85 | 95.20 | 34.81 |
| 14.87 | 93.11 | 34.84 |
| 14.89 | 88.61 | 34.25 |
| 14.92 | 81.84 | 32.51 |
| 14.94 | 73.54 | 31.18 |
| 14.97 | 64.28 | 29.91 |
| 14.99 | 54.65 | 28.31 |
| 15.02 | 45.39 | 27.15 |
| 15.04 | 36.74 | 26.37 |
| 15.06 | 29.31 | 26.30 |
| 15.09 | 23.31 | 27.59 |
| 15.11 | 18.60 | 29.29 |
| 15.14 | 14.85 | 32.40 |
| 15.16 | 12.24 | 37.32 |
| 15.18 | 10.72 | 42.31 |
| 15.21 | 9.92 | 48.80 |
| 15.23 | 9.85 | 55.12 |
| 15.26 | 9.50 | 61.48 |
| 15.28 | 9.94 | 67.53 |
| 15.30 | 9.92 | 72.08 |
| 15.33 | 9.95 | 75.20 |
| 15.35 | 10.25 | 76.03 |
| 15.38 | 10.08 | 75.04 |
| 15.40 | 9.57 | 71.51 |
| 15.42 | 9.36 | 66.80 |
| 15.45 | 8.75 | 60.38 |
| 15.47 | 8.00 | 53.11 |
| 15.50 | 7.17 | 45.22 |
| 15.52 | 6.24 | 37.54 |
| 15.54 | 5.53 | 29.93 |
| 15.57 | 4.41 | 23.39 |
| 15.59 | 3.64 | 17.80 |
| 15.62 | 3.11 | 12.88 |
| 15.64 | 2.57 | 9.75 |
| 15.66 | 2.00 | 6.77 |
| 15.69 | 1.39 | 4.09 |
| 15.71 | 0.99 | 2.76 |
| 15.74 | 0.98 | 1.73 |
| 15.76 | 0.69 | 1.34 |
| 15.78 | 0.55 | 0.62 |
| 15.81 | 0.19 | 0.60 |
| 15.83 | 0.06 | 0.18 |
| 15.86 | 0.11 | 0.00 |
| 15.88 | 0.24 | -0.21 |
| 15.90 | 0.23 | 0.04 |
| 15.93 | -0.09 | -0.18 |
| 15.95 | 0.07 | -0.17 |
| 15.98 | 0.19 | -0.32 |
| 16.00 | 0.09 | 0.11 |
| 16.02 | 0.15 | -0.37 |
| 16.05 | -0.01 | 0.15 |
| 16.07 | 0.19 | -0.01 |
| 16.10 | -0.03 | 0.12 |
| 16.12 | 0.04 | -0.05 |
| 16.14 | -0.18 | 0.17 |
| 16.17 | 0.05 | -0.07 |
| 16.19 | 0.18 | -0.07 |
| 16.22 | -0.06 | 0.26 |
| 16.24 | 0.16 | -0.02 |
| 16.26 | 0.20 | 0.04 |
| 16.29 | -0.14 | 0.11 |
| 16.31 | 0.16 | 0.35 |
| 16.34 | 0.12 | -0.13 |
| 16.36 | -0.08 | -0.06 |
| 16.38 | 0.16 | 0.20 |
| 16.41 | 0.04 | -0.11 |
| 16.43 | 0.14 | -0.11 |
| 16.46 | 0.04 | -0.11 |
| 16.48 | -0.08 | 0.08 |
| 16.50 | 0.17 | 0.23 |
| 16.53 | 0.15 | 0.02 |
| 16.55 | -0.09 | 0.11 |
| 16.58 | 0.13 | -0.38 |
| 16.60 | -0.07 | 0.02 |
| 16.62 | -0.07 | 0.03 |
| 16.65 | 0.11 | 0.03 |
| 16.67 | 0.10 | 0.07 |
| 16.70 | 0.04 | -0.08 |
| 16.72 | 0.13 | -0.07 |
| 16.74 | 0.02 | -0.05 |
| 16.77 | -0.03 | -0.01 |
| 16.79 | -0.01 | 0.14 |
| 16.82 | -0.18 | -0.27 |
| 16.84 | -0.04 | 0.01 |
| 16.86 | -0.19 | -0.28 |
| 16.89 | 0.00 | -0.01 |
| 16.91 | -0.20 | 0.17 |
| 16.94 | 0.11 | -0.07 |
| 16.96 | -0.02 | 0.03 |
| 16.98 | 0.19 | -0.02 |
| 17.01 | -0.19 | 0.01 |
| 17.03 | -0.19 | 0.15 |
| 17.06 | -0.10 | 0.08 |
| 17.08 | 0.14 | -0.07 |
| 17.11 | -0.17 | 0.01 |
| 17.13 | 0.05 | -0.09 |
| 17.15 | -0.21 | -0.22 |
| 17.18 | -0.06 | 0.31 |
| 17.20 | -0.08 | 0.22 |
| 17.23 | -0.06 | 0.07 |
| 17.25 | 0.21 | -0.19 |
| 17.27 | -0.14 | -0.08 |
| 17.30 | 0.10 | 0.01 |
| 17.32 | 0.05 | 0.02 |
| 17.35 | 0.08 | -0.02 |
| 17.37 | 0.07 | -0.07 |
| 17.39 | -0.08 | -0.16 |
| 17.42 | 0.19 | -0.11 |
| 17.44 | -0.16 | -0.21 |
| 17.47 | 0.14 | 0.21 |
| 17.49 | 0.05 | -0.04 |
| 17.51 | -0.14 | -0.05 |
| 17.54 | 0.17 | 0.28 |
| 17.56 | 0.19 | 0.25 |
| 17.59 | 0.20 | 0.17 |
| 17.61 | -0.15 | 0.22 |
| 17.63 | 0.19 | 0.18 |
| 17.66 | 0.07 | 0.22 |
| 17.68 | 0.16 | 0.26 |
| 17.71 | 0.16 | 0.05 |
| 17.73 | -0.05 | 0.32 |
| 17.75 | -0.05 | -0.05 |
| 17.78 | 0.00 | 0.03 |
| 17.80 | 0.02 | -0.10 |
| 17.83 | 0.12 | -0.15 |
| 17.85 | 0.15 | -0.03 |
| 17.87 | 0.07 | -0.34 |
| 17.90 | 0.10 | -0.01 |
| 17.92 | 0.00 | -0.10 |
| 17.95 | -0.18 | 0.23 |
| 17.97 | -0.07 | 0.29 |
| 17.99 | 0.00 | -0.19 |
| 18.02 | 0.01 | -0.06 |
| 18.04 | -0.16 | -0.07 |
| 18.07 | 0.00 | -0.16 |
| 18.09 | 0.08 | 0.20 |
| 18.11 | 0.18 | 0.22 |
| 18.14 | -0.05 | -0.05 |
| 18.16 | -0.09 | 0.37 |
| 18.19 | 0.00 | -0.04 |
| 18.21 | 0.06 | 0.11 |
| 18.23 | -0.17 | -0.18 |
| 18.26 | 0.08 | -0.05 |
| 18.28 | 0.03 | -0.23 |
| 18.31 | 0.09 | -0.07 |
| 18.33 | -0.10 | -0.20 |
| 18.35 | -0.14 | -0.02 |
| 18.38 | -0.13 | 0.35 |
| 18.40 | 0.03 | 0.05 |
| 18.43 | -0.03 | 0.19 |
| 18.45 | -0.11 | -0.16 |
| 18.47 | 0.12 | -0.26 |
| 18.50 | -0.03 | -0.35 |
| 18.52 | 0.15 | -0.03 |
| 18.55 | 0.06 | -0.03 |
| 18.57 | -0.18 | -0.15 |
| 18.59 | 0.11 | 0.13 |
| 18.62 | 0.12 | 0.12 |
| 18.64 | 0.02 | 0.21 |
| 18.67 | -0.04 | -0.19 |
| 18.69 | -0.09 | -0.01 |
| 18.71 | 0.10 | -0.21 |
| 18.74 | 0.04 | 0.01 |
| 18.76 | 0.06 | 0.17 |
| 18.79 | -0.03 | 0.03 |
| 18.81 | -0.05 | -0.27 |
| 18.83 | 0.09 | -0.03 |
| 18.86 | 0.08 | -0.30 |
| 18.88 | -0.19 | 0.01 |
| 18.91 | 0.20 | -0.12 |
| 18.93 | 0.09 | 0.20 |
| 18.95 | -0.05 | -0.27 |
| 18.98 | 0.16 | 0.19 |
| 19.00 | -0.15 | 0.18 |
| 19.03 | 0.19 | -0.15 |
| 19.05 | -0.16 | 0.21 |
| 19.08 | 0.10 | 0.01 |
| 19.10 | -0.17 | 0.04 |
| 19.12 | -0.06 | -0.13 |
| 19.15 | 0.03 | -0.17 |
| 19.17 | -0.13 | 0.16 |
| 19.20 | -0.08 | 0.01 |
| 19.22 | -0.07 | 0.02 |
| 19.24 | -0.03 | 0.08 |
| 19.27 | 0.01 | 0.22 |
| 19.29 | 0.07 | 0.17 |
| 19.32 | 0.03 | 0.19 |
| 19.34 | -0.18 | -0.30 |
| 19.36 | 0.12 | 0.23 |
| 19.39 | 0.14 | 0.06 |
| 19.41 | 0.01 | -0.10 |
| 19.44 | -0.10 | -0.33 |
| 19.46 | 0.13 | -0.05 |
| 19.48 | 0.00 | 0.11 |
| 19.51 | 0.19 | -0.15 |
| 19.53 | -0.12 | 0.17 |
| 19.56 | 0.14 | 0.10 |
| 19.58 | -0.06 | -0.13 |
| 19.60 | -0.14 | -0.17 |
| 19.63 | 0.00 | 0.15 |
| 19.65 | 0.10 | 0.25 |
| 19.68 | 0.02 | 0.02 |
| 19.70 | 0.09 | 0.30 |
| 19.72 | -0.03 | 0.01 |
| 19.75 | -0.13 | -0.16 |
| 19.77 | -0.17 | 0.19 |
| 19.80 | -0.06 | -0.09 |
| 19.82 | -0.14 | 0.12 |
| 19.84 | 0.06 | 0.24 |
| 19.87 | 0.01 | 0.25 |
| 19.89 | 0.02 | -0.18 |
| 19.92 | -0.16 | 0.25 |
| 19.94 | -0.12 | -0.16 |
| 19.96 | 0.15 | 0.12 |
| 19.99 | -0.04 | -0.25 |
| 20.01 | 0.16 | -0.20 |
| 20.04 | 0.05 | -0.15 |
| 20.06 | -0.06 | 0.15 |
| 20.08 | -0.05 | -0.03 |
| 20.11 | 0.07 | -0.18 |
| 20.13 | -0.18 | 0.01 |
| 20.16 | 0.08 | 0.00 |
| 20.18 | 0.03 | 0.24 |
| 20.20 | -0.10 | -0.32 |
| 20.23 | 0.02 | -0.12 |
| 20.25 | -0.08 | 0.03 |
| 20.28 | -0.05 | -0.08 |
| 20.30 | -0.09 | 0.12 |
| 20.32 | -0.04 | 0.02 |
| 20.35 | -0.15 | 0.09 |
| 20.37 | 0.10 | -0.23 |
| 20.40 | 0.21 | -0.29 |
| 20.42 | -0.16 | -0.07 |
| 20.44 | -0.02 | 0.00 |
| 20.47 | -0.19 | -0.30 |
| 20.49 | 0.11 | -0.17 |
| 20.52 | 0.03 | -0.08 |
| 20.54 | 0.19 | 0.00 |
| 20.56 | -0.16 | 0.13 |
| 20.59 | 0.14 | 0.07 |
| 20.61 | 0.15 | 0.08 |
| 20.64 | 0.15 | -0.10 |
| 20.66 | 0.07 | 0.06 |
| 20.68 | 0.16 | -0.06 |
| 20.71 | 0.03 | 0.10 |
| 20.73 | -0.09 | 0.16 |
| 20.76 | 0.21 | 0.17 |
| 20.78 | 0.06 | -0.30 |
| 20.80 | 0.12 | 0.23 |
| 20.83 | -0.09 | -0.20 |
| 20.85 | -0.09 | 0.07 |
| 20.88 | 0.07 | 0.01 |
| 20.90 | -0.10 | 0.23 |
| 20.92 | -0.01 | 0.13 |
| 20.95 | -0.05 | 0.03 |
| 20.97 | 0.09 | 0.02 |
| 21.00 | 0.14 | 0.14 |
| 21.02 | 0.01 | 0.28 |
| 21.05 | 0.03 | -0.12 |
| 21.07 | -0.01 | -0.13 |
| 21.09 | -0.06 | -0.08 |
| 21.12 | 0.06 | 0.21 |
| 21.14 | 0.15 | -0.08 |
| 21.17 | -0.01 | -0.25 |
| 21.19 | 0.01 | -0.12 |
| 21.21 | 0.15 | 0.32 |
| 21.24 | -0.10 | 0.07 |
| 21.26 | 0.05 | 0.21 |
| 21.29 | -0.01 | 0.03 |
| 21.31 | -0.10 | 0.10 |
| 21.33 | 0.11 | 0.12 |
| 21.36 | -0.08 | -0.03 |
| 21.38 | -0.11 | -0.13 |
| 21.41 | -0.15 | -0.21 |
| 21.43 | -0.09 | 0.01 |
| 21.45 | 0.16 | -0.08 |
| 21.48 | -0.10 | -0.10 |
| 21.50 | -0.01 | -0.06 |
| 21.53 | -0.16 | -0.13 |
| 21.55 | -0.19 | 0.03 |
| 21.57 | -0.06 | 0.16 |
| 21.60 | -0.04 | 0.05 |
| 21.62 | -0.14 | -0.21 |
| 21.65 | 0.10 | -0.01 |
| 21.67 | -0.13 | -0.14 |
| 21.69 | -0.14 | 0.12 |
| 21.72 | 0.11 | -0.19 |
| 21.74 | -0.01 | -0.04 |
| 21.77 | -0.01 | 0.04 |
| 21.79 | -0.01 | 0.22 |
| 21.81 | -0.03 | 0.31 |
| 21.84 | 0.14 | 0.03 |
| 21.86 | -0.04 | -0.03 |
| 21.89 | -0.07 | 0.01 |
| 21.91 | -0.16 | 0.29 |
| 21.93 | -0.08 | -0.15 |
| 21.96 | -0.13 | -0.03 |
| 21.98 | -0.19 | 0.18 |
| 22.01 | 0.17 | -0.30 |
| 22.03 | 0.09 | 0.27 |
| 22.05 | 0.14 | -0.35 |
| 22.08 | -0.18 | -0.05 |
| 22.10 | 0.09 | 0.01 |
| 22.13 | -0.17 | 0.13 |
| 22.15 | -0.03 | 0.16 |
| 22.17 | -0.15 | 0.10 |
| 22.20 | 0.11 | 0.11 |
| 22.22 | 0.12 | 0.05 |
| 22.25 | -0.14 | 0.03 |
| 22.27 | 0.16 | 0.24 |
| 22.29 | -0.09 | -0.11 |
| 22.32 | -0.02 | -0.23 |
| 22.34 | 0.21 | -0.14 |
| 22.37 | -0.17 | -0.12 |
| 22.39 | 0.06 | -0.01 |
| 22.41 | 0.16 | 0.13 |
| 22.44 | -0.16 | 0.25 |
| 22.46 | -0.09 | 0.14 |
| 22.49 | 0.06 | -0.28 |
| 22.51 | 0.10 | 0.23 |
| 22.53 | -0.18 | -0.21 |
| 22.56 | 0.17 | -0.26 |
| 22.58 | -0.15 | 0.07 |
| 22.61 | 0.02 | 0.20 |
| 22.63 | -0.20 | -0.18 |
| 22.65 | 0.08 | 0.00 |
| 22.68 | -0.20 | -0.27 |
| 22.70 | -0.05 | 0.20 |
| 22.73 | -0.07 | -0.19 |
| 22.75 | -0.05 | -0.10 |
| 22.77 | 0.02 | 0.17 |
| 22.80 | 0.11 | -0.08 |
| 22.82 | 0.10 | 0.04 |
| 22.85 | -0.03 | -0.11 |
| 22.87 | -0.05 | 0.03 |
| 22.89 | -0.19 | 0.15 |
| 22.92 | -0.12 | -0.01 |
| 22.94 | 0.16 | -0.07 |
| 22.97 | -0.04 | -0.32 |
| 22.99 | -0.13 | 0.21 |
| 23.02 | 0.06 | -0.23 |
| 23.04 | -0.10 | -0.30 |
| 23.06 | 0.09 | -0.22 |
| 23.09 | 0.05 | -0.33 |
| 23.11 | -0.09 | -0.21 |
| 23.14 | -0.14 | -0.03 |
| 23.16 | -0.17 | -0.06 |
| 23.18 | 0.15 | -0.20 |
| 23.21 | 0.04 | 0.05 |
| 23.23 | 0.09 | -0.34 |
| 23.26 | 0.19 | 0.25 |
| 23.28 | 0.20 | -0.10 |
| 23.30 | 0.16 | -0.18 |
| 23.33 | 0.05 | -0.03 |
| 23.35 | 0.06 | -0.08 |
| 23.38 | -0.17 | 0.19 |
| 23.40 | -0.09 | 0.17 |
| 23.42 | 0.03 | 0.35 |
| 23.45 | 0.14 | 0.06 |
| 23.47 | -0.09 | 0.17 |
| 23.50 | -0.02 | -0.25 |
| 23.52 | -0.04 | 0.03 |
| 23.54 | 0.01 | 0.00 |
| 23.57 | -0.11 | -0.10 |
| 23.59 | -0.02 | 0.15 |
| 23.62 | -0.20 | 0.15 |
| 23.64 | -0.11 | -0.16 |
| 23.66 | 0.13 | 0.12 |
| 23.69 | 0.17 | -0.14 |
| 23.71 | -0.13 | -0.26 |
| 23.74 | 0.19 | 0.09 |
| 23.76 | 0.11 | -0.04 |
| 23.78 | -0.16 | 0.32 |
| 23.81 | 0.19 | 0.05 |
| 23.83 | -0.14 | 0.21 |
| 23.86 | -0.05 | 0.13 |
| 23.88 | 0.15 | -0.09 |
| 23.90 | 0.17 | 0.21 |
| 23.93 | -0.17 | 0.13 |
| 23.95 | -0.10 | -0.07 |
| 23.98 | -0.11 | 0.21 |
| 24.00 | -0.05 | 0.02 |
This Document: 124
README: 50
Total: 174
Report made with rmarkdown (Allaire, Xie, McPherson, et al., 2021; Xie, Allaire, and Grolemund, 2018; Xie, Dervieux, and Riederer, 2020), RefManageR(McLean, 2020).
Theme is Minty by Bootswatch.
5 Bibliography
Packages
Allaire, J., Y. Xie, J. McPherson, et al. (2021). rmarkdown: Dynamic Documents for R. R package version 2.11. URL: https://CRAN.R-project.org/package=rmarkdown.
McLean, M. W. (2020). RefManageR: Straightforward BibTeX and BibLaTeX Bibliography Management. R package version 1.3.0. URL: https://github.com/ropensci/RefManageR/.
Xie, Y., J. Allaire, and G. Grolemund (2018). R Markdown: The Definitive Guide. ISBN 9781138359338. Boca Raton, Florida: Chapman and Hall/CRC. URL: https://bookdown.org/yihui/rmarkdown.
Xie, Y., C. Dervieux, and E. Riederer (2020). R Markdown Cookbook. ISBN 9780367563837. Boca Raton, Florida: Chapman and Hall/CRC. URL: https://bookdown.org/yihui/rmarkdown-cookbook.
References
d’Adda di Fagagna, F., G. R. Weller, A. J. Doherty, et al. (2003). “The Gam protein of bacteriophage Mu is an orthologue of eukaryotic Ku”. En. In: EMBO reports 4.1, pp. 47-52.